Hyperspectral Microscopy Technology to Detect Syrups Adulteration of Endemic Guindo Santo and Quillay Honey Using Machine-Learning Tools.

Honey adulteration is a common practice that affects food quality and sale prices, and certifying the origin of the honey using non-destructive methods is critical. Guindo Santo and Quillay are fundamental for the honey production of Biobío and the Ñuble region in Chile. Furthermore, Guindo Santo only exists in this area of the world. Therefore, certifying honey of this species is crucial for beekeeper communities-mostly natives-to give them advantages and competitiveness in the global market. To solve this necessity, we present a system for detecting adulterated endemic honey that combines different artificial intelligence networks with a confocal optical microscope and a tunable optical filter for hyperspectral data acquisition. Honey samples artificially adulterated with syrups at concentrations undetectable to the naked eye were used for validating different artificial intelligence models. Comparing Linear discriminant analysis (LDA), Support vector machine (SVM), and Neural Network (NN), we reach the best average accuracy value with SVM of 93% for all classes in both kinds of honey. We hope these results will be the starting point of a method for honey certification in Chile in an automated way and with high precision.

Carnosine-like immunoreactivity in neurons of the brain of an advanced teleost, the gray mullet (Chelon labrosus, Risso).

The distribution of the dipeptide carnosine (beta-alanyl-L-histidine) and the related dipeptides anserine and homocarnosine has been studied by biochemical methods and immunohistochemistry in the brain of mammals and other tetrapods. These studies have indicated the presence of these dipeptides mainly in glial cells and in some neurons (olfactory receptor neurons and certain putative migrating neurons and neuroblasts). Here, we used immunohistochemistry with a polyclonal anti-carnosine antibody and the streptavidin-avidin method to study for the first time the distribution of carnosine/carnosine-related dipeptides in the brain of a teleost fish (the gray mullet Chelon labrosus). In order to assess the neuronal nature of carnosine-immunoreactive cells, we also used double immunofluorescence methods with antibodies to carnosine and to the neuronal protein HuC/D. The results obtained show that carnosine and/or related dipeptides are present in neurons of various brain regions. The carnosine-like-immunoreactive neuronal populations of the optic tectum and cerebellum are described in detail. In the optic tectum, immunoreactivity to carnosine/carnosine-related dipeptides is present in neurons of the stratum album and the stratum griseum centrale. In the cerebellum, immunoreactivity to these dipeptides is localized in Purkinje cells, in putative Golgi and stellate cells, and in many nerve fibers. Carnosine-like immunoreactive cells in mullet brain seem to be specific types of neurons, in line with previous results in a urodele but at variance with previous results in mammals, reptiles and anurans.

Calretinin immunoreactivity in taste buds and afferent fibers of the grey mullet Chelon labrosus.

The presence of the calcium-binding protein calretinin in taste buds of a teleost, the thick-lipped grey mullet, was investigated using immunohistochemical techniques. Taste bud sensory cells had calretinin immunoreactivity. The nerve fiber plexus innervating taste buds, the ganglia and the viscerosensory roots projecting to the vagal lobe, also showed calretinin immunoreactivity. These results demonstrate for the first time the occurrence of calretinin in the taste buds and the taste afferent system of a teleost.